Our scientists are professional in construction of various phage display libraries, including cDNA libraries, peptide libraries and scFv/Fab antibody libraries. We normally generate libraries with a diversity of n×108; we are able to generate libraries of n×109-10. In case you need a special cDNA, peptide or antibody library, you can rely on our extensive experience. For peptide libraries with inserts longer than 8-mer (and up to 30-mer), we usually use one of three nucleotide doping strategies (NNK, Split-mix-split synthesis or Tri-mer codon) to maximize the amino acid sequences encoded by the inserted DNA sequences. We can make the both pIII-fusion libraries and pVIII-fusion libraries. For scFv/Fab library construction, we can use both human plasma cells and splenocytes from native or immunized mice. We are also professional in producing fully or semi-synthetic antibody libraries.
Phage display systems can be grouped into two classes based on the vector system.
Library types:
- Phage display peptide libraries;
- Phage display antibody libraries, including immunized antibody libraries, native antibody libraries and semi-synthetic/synthetic antibody libraries.
Construction vectors:
- New England BioLab’s true phage vector, M13KE, for construction of peptide library;
- pIII fusion phagemid for construction of peptide library and pVIII fusion phagemid for construction of peptide library;
- Novagen’s T7Select Phage Display vector for construction of cDNA library.
Diversity of synthetic libraries:
- “Hard randomization”: complete diversity is introduced by using degenerate codons encoding all twenty natural amino acids
- “Tailored randomization”: incomplete or biased diversity is introduced by using degenerate codons encoding a subset of natural amino acids
- Diversification: degenerate codons are synthesized using trimer phosphoramidites corresponding to 20 natural amino acids, which exclude stop codons.
Library capacity:
Large phage display libraries containing 107-10 variants