Biopanning

Description

This process is an affinity selection technique which selects for peptides that bind to a given target.

Our process involves 4 major steps for peptide selection. The first step is to have phagedisplay libraries prepared. This involves inserting foreign desired gene segments into a region of the bacteriophage genome, so that the peptide product will be displayed on the surface of the bacteriophage virion. The most often used are genes pIII or pVIII of bacteriophage M13 The next step is the capturing step. It involves conjugating the phage library to the desired target. This procedure is termed panning. It utilizes the binding interactions so that only specific peptides presented by bacteriophage are bound to the target. For example, selecting antibody presented by bacteriophage with coated antigen in microtiter plates.

The end result is the peptides produced by bacteriophage are specific. The resulting filamentous phages can infect Gram negative bacteria once again to produce phage libraries. The cycle can occur many times resulting with strong affinity binding peptides to the target.