Inmunization

Description

A vaccine is a biological preparation that provides active acquired immunity to a particular disease. A vaccine typically contains an agent that resembles a causative organism of the disease and is often made from dead or weakened microbes forms, their toxins or one of its surface proteins. The drug stimulates the body’s immune system to recognize the agent as a threat, destroying it and keep a record of it, so that the immune system can recognize and more easily destroy any of these microorganisms to find later.

What we emulsion concentration?

The usual concentration for injection into a rat or mouse is 50 to 100 g. For a rabbit concentration ranges from 50 to 1000.mu. g. Before injection must be diluted with saline precipitate in a final volume of 250 uL.

We want to immunize a mouse with 100 ug BSA What volume of emulsion need? and saline?

Is required:

• Prepare 10 ml of a 5% solution of potassium sulphate Alluminium (potassium aluminum sulfate) [KAl (S04) 2.12 H20] in sterile distilled water. It is known as Alum.
• Prepare 1 ml of antigen (BSA) diluted to a concentration of 1 mg / ml in distilled water in a 15 ml Falcon tube.
• Add 1 ml of 5% solution alumn. Adjust the pH to 6.5 by adding 4N NaOH slowly. Usually it requires several aliquots of 30 ul, although this varies according to the pH of the water. Measuring the pH with indicator paper.
• Centrifuge sample 5 minutes at maximum centrifugation in a centrifuge (13,000 rpm) Remove the supernatant with a Pasteur pipette.
• Resuspend the pellet in 1 ml sterile saline (final concentration of 1 mg / ml) Keep at 4 ° C. If you would like to keep many days (at least 14), resuspend in saline / merthiolate 1: 10,000.

IMMUNIZATION ROUTES

The choice of route of immunization will depend on several factors:

Hade amount administered, solution in which resuspended or diluted to the immunogen and what other components are associated (e.g., B. pertussis cannot inoculated via ntravenosa) and the speed or rate at which the immunogen should be released lymphatic vessels and circulation.

The main routes of immunization are:

Subcutaneous or hypodermic, intramuscular, intradermal, intravenous, lymphoid nodules.

Our Intraperitoneal process uses frequently in small animals (mouse and rat) allows inoculate a large volume in mice, it is not recommended for rabbits

 

MORE ANIMALS USED

Animal experimentation is one of the most important pillars of scientific research. Working with animals allows for reliable and economic experimental models, but ethically requires the researcher to avoid the production of unnecessary pain to the animal. The principal animal species used in experiments are:

Rabbits, mice, rats, chickens, Human.

Since the response of the animals will be different, they should immunize several individuals. If immunizing rabbits should be at least two, still better 3 or 4. In the case of rodents should be used between 3 to 6 animals.